GATE BT 2012 Answer key
Q1.In mismatch correction repair, the parental DNA strand is distinguished from the daughter strand by
Answer:(C) (Methylation)
Q2.The basis for blue-white screening with pUC vectors is
Answer:(A) Intraallelic complementation
Q3.Idiotypic determinants of an antibody are associated with the
Answer:(C) Variable region
Q4.Identification of blood groups involves
Answer:(D) Agglutination
Q5.B-lymphocytes originate from the bone marrow whereas T-lymphocytes originate from
Answer:(B) Bone marrow
Q6.A humanized antibody is one in which the
Answer:(D) CDRs are from mouse, and the rest is from human
Q7.Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because
Answer:(C) It protects cells by preventing crystallization of water
Q8.Nude mice refers to
Answer:(B) Mice without thymus
Q9.Heat inactivation of serum is done to inactivate
Answer:(C) Complement
Q10.Choose the correct signal transduction pathway
Answer:(A) Hormone 7 TM receptor Gprotein cAMP PKA
Q11.A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine with
Answer:(D) Threonine
Q12.A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide?
Answer:(B) Transfer RNA gene
Q13.Protein-DNA interactions in vivo can be studied by
Answer:(D) Fluorescence in situ hybridization assay
Q14.The direction of shell coiling in the snail Limnaea peregra is a classic example of
Answer:(B) Extra-chromosomal inheritance
Q15.During photorespiration under low CO2 and high O2 levels, O2 reacts with ribulose 1,5- bisphosphate to yield
Answer:(D) One molecule each of 3-phosphoglycerate and glyoxylate
Q16.Which one of the following is NOT a protoplast fusion inducing agent?
Answer:(D) Colchicine
Q17.The activity of an enzyme is expressed in International Units (IU). However, the S.I. unit for enzyme activity is Katal. One Katal is
Answer:(C) 6 ×10 IU
Q18.Identify the statement that is NOT applicable to an enzyme catalyzed reaction
Answer:(C) Enzymes do not accelerate the rate of reverse reaction
Q19.An example of a derived protein structure database is
Answer:(A) Pfam
Q20.An example of a program for constructing a phylogenetic tree is
Answer:(A) Phylip
Q21.Synteny refers to
Answer:(A) Gene duplication from a common ancestor
Q22.While searching a database for similar sequences, E value does NOT depend on the
Answer:(D) Probability from a normal distribution
Q23.In transmission electron microscopy, election opacity is greatly enhanced by treating the specimen with
Answer:(B) Uranium acetate
Q24.The molarity of water in a water: ethanol mixture (15: 85, v/v) is approximately
Answer:(C) 8.5
Q25.The helix content of a protein can be determined using
Answer:(C) A circular dichroism spectrometer
Q26.Which one of the following DNA sequences carries an invert repeat?
Answer:(A) ATGAGCCCCGAGTA
TACTCGGGGCTCAT
Q27.In zinc finger proteins, the amino acid residues that coordinate zinc are
Answer:(A) Cys and His
Q28.Match the entries in Group I with those in Group II.
Group I Group II
P. MTT 1. Dihydrofolate reductase
Q. Annexin V 2. Succinate dehydrogenase
R. Methotrexate 3. Microtubules
S. Taxol 4. Phosphatidylserine
Answer:(B) P - 2,Q - 4,R - 1,S - 3
Q29.In an exponentially growing batch culture of Saccharomyces cerevisiae, the cell density is 1 20gl- (DCW), the specific growth rate (µ) is 0.4 and substrate uptake rate (v) is 1 1 16 g/lh. The cell yield coefficient Yx/s will be
Answer:(B) 0.64
Q30.A single base pair of DNA weighs 1.1x10*21 grams. How many picomoles of a plasmid vector of length 2750 bp are contained in 1µg of purified DNA?
Answer:(B) 0.55
Q31.Match the terms in Group I with the ploidy in Group II.
Group I Group II
P. Disome 1. 2n + 1
Q. Monosome 2. 2n – 1
R. Nullisome 3. n – 1
S. Trisome 4. n + 1
Answer:(A) P - 4,Q - 2,R - 3,S - 1
Q32.What is the rank of the following matrix?
5 3 1
6 2 4
14 10 0
Answer:(C) 2
Q33.Match the products in Group I with the applications in Group II
Group I Group II
P. Digoxin 1. Muscle relaxant
Q. Stevioside 2. Anti cancer agent
R. Atropine 3. Cardiovascular disorder
S. Vinblastine 4. Sweetener
Answer:(C) P - 3,Q - 4,R - 1,S - 2
Q34.Determine the correctness or otherwise of the following Assertion (a) and Reason(r).
Assertion : The production of secondary metabolites in plant cell cultures is enhanced by the addition of elicitors
Reason : Elicitors induce the expression of enzymes responsible for the biosynthesis of secondary metabolites
Answer:(C) (a) is true but (r) is false
Q35.Determine the correctness or otherwise of the following Assertion (a) and Reason(r).
Assertion : Plants convert fatty acids into glucose
Reason : Plants have peroxisomes
Answer:(D) (D) (a) is false but (r) is true
Q36.Determine the correctness or otherwise of the following Assertion (a)and Reason (r).
Assertion : In direct somatic embryogenesis, embryos are developed without going through callus formation
Reason : This is possible due to the presence of pre-embryonically determined cells
Answer:(D) Both (a) and (r) are true and (r) is the correct reason for (a)
Q37.Match the entries in Group I with the process parameters in Group II
Group I Group II
P. Clark electrode 1. Liquid level
Q. Redox probe 2. Dissolved oxygen concentration
R. Load cell 3. Vessel pressure
S. Diaphragm gauge 4. pH (anaerobic process)
Answer:(C) P - 2,Q - 4,R - 1,S - 3
Q38.Match the downstream processes in Group I with the products in Group II.
Group I Group II
P. Solvent extraction 1. Lactic acid
Q. Protein-A linked affinity chromatography 2. Penicillin
R. Extractive distillation 3. Monoclonal antibody
S. Salting out 4. Lipase
Answer:(A) P - 2,Q - 3,R - 1,S - 4
Q39.Determine the correctness or otherwise of the following Assertion (a) and Reason(r).
Assertion : Cell mass yield of methylotrophic yeast is more on methanol compared to glucose
Reason : Methanol has a greater degree of reductance compared to glucose.
Answer:(D) Both (a) and (r) are correct but (r) is not the correct reason for (a)
Q40.A disease is inherited by a child with a probability of 1/4. In a family with two
children, the probability that exactly one sibling is affected by this disease is
Answer:(B) 3/8
Q41.Match the organisms in Group I with the entries in Group II.
Group I Group II
P. Clostridium 1. Rods with teichoic acid in the cell wall
Q. Escherichia 2. Rods with endospores
R. Vibrio 3. Helical rods with flagella
S. Bacillus 4. Rods with LPS in the outer membrane
5. Curved rods with polar flagella
Answer:(A) P - 2,Q - 4,R - 5,S - 1
Q42.Match the entries in Group I with the methods of sterilization in Group II.
Group I Group II
P. Serum 1. Autoclave
Q. Luria broth 2. Membrane filtration
R. Polypropylene tubes 3. UV irradiation
S. Biological safety cabinets 4. Gamma irradiation
5. Dry heat
Answer:(C) P - 2,Q - 1,R - 4,S - 3
Q43.Match the high energy compounds in Group I with the biosynthetic pathways for the
molecules in Group II.
Group I Group II
P. GTP 1. Fatty acid
Q. UTP 2. Phospholipid
R. CTP 3. Protein
S. Acyl coenzyme A 4. Peptidoglycan
Answer:(D) P - 3,Q - 4,R - 2,S - 1
Q44.Match the vitamins in Group I with the processes/reactions in Group II.
Group I Group II
P. Pantothenic acid 1. Electron transport
Q. Vitamin B2 2. Transfer of 1-C units
R. Vitamin B6 3. Decarboxylation
S. Folic acid 4. Fatty acid metabolism
5. Hydrolysis
Answer:(B) P - 4,Q - 1,R - 3,S - 2
Q45.Consider the data set 14, 18, 14, 14, 10, 29, 33, 31, 25. If you add 20 to each of
the values, then
Answer:(D) The mean changes, the variance is unchanged
Q46.An enzymatic reaction is described by the following rate expression
Answer:(A)
Q47.A bacterial culture ( ) 9 200 l µ containing 1.8 ×10 cells was treated with an antibiotic Z (50µg per ml) for 4 h at 37oC. After this treatment, the culture was
divided into two equal aliquots.
Set A: 100µl was plated on Luria agar.
Set B: 100µl was centrifuged, the cell pellet washed and plated on Luria agar.
After incubating these two plates for 24 h at 37°C, Set A plate showed no
colonies, whereas the Set B plate showed 9 0.9 ×10 cells. This experiment showed
that the antibiotic Z is
Answer:(A) Bacteriostatic
Common Data Questions: 48 & 49
In a muscle, the extracellular and intracellular concentrations of Na+ are 150 mM
and 12 mM and those of K+ are 2.7 mM and 140 mM, respectively. Assume that
the temperature is 25oC and that the membrane potential is –60mV, with the
interior more negatively charged than the exterior.
Q48.The free energy change for the transport of three Na+ out of the cell is
Answer:(D) +36.3 kJ /mol
Q49.The free energy change for the transport of two K+ into the cell is
Answer:(A) +8.0 kJ /mol
Common Data Questions: 50 & 51
The purification data for an enzyme is given below:
Q50.The fold purification for each step is
Answer:(C) P - 1,Q - 6.6,R - 8.4,S - 12.6
Q51.The yield (%) for each step is
Answer:(D) P - 100,Q - 71,R - 44,S - 29
Linked Answer Questions: Q.52 to Q.55 Carry Two Marks Each
Statement for Linked Answer Questions: 52 & 53
An E. coli cell of volume 12 3 10 cm - contains 60 molecules of lac-repressor. The
repressor has a binding affinity ( ) 8 9
d K of 10 M and 10 M with and without lactose
respectively, in the medium
Q52.The molar concentration of the repressor in the cell is
Answer:(D) 100 nM
Q53.Therefore the lac-operon is
Answer:(A) Repressed and can only be induced with lactose
Statement for Linked Answer Questions: 54 & 55
b- Galactosidase bound to DEAE-cellulose is used to hydrolyze lactose to glucose
and galactose in a plug flow bioreactor with a packed bed of volume 100 liters
and a voidage (e) of 0.55. The Km and Vmax for the immobilized enzyme are
1 1 1 0.72 gl and 18gl h , respectively. The lactose concentration in the field stream
is 1 20gl-and a fractional conversion of 0.90 is desired. Diffusional limitations
may be ignored.
Q54.The residence time required for the steady state reactor operation will be
Answer:(C) 1.0 h
Q55.The feed flow rate required for the above bioconversion wi l l be
Answer: (A) 1 50 lh-1
Q56.The cost function for a product in a firm is given by 2 5q , where q is the amount
of production. The firm can sell the product at a market price of Rs.50 per unit.
The number of uni ts to be produced by the firm such that the profit is
maximized is
Answer:(A) 5
Q57.Choose the most appropriate alternative from the options given below to
complete the following sentence:
Suresh's dog is the one ________ was hurt in the stampede.
Answer:(A)that
Q58.Choose the grammatically INCORRECT sentence:
(A) They gave us the money back less the service charges of Three Hundred rupees.
(B) This country's expenditure is not less than that of Bangladesh.
(C) The committee initially asked for a funding of Fifty Lakh rupees, but later settled for a lesser sum.
(D) This country's expenditure on educational reforms is very less
Answer:(D) This country's expenditure on educational reforms is very less
Q59. Which one of the following options is the closest in meaning to the word given
below?
Mitigate
Answer:(A) Diminish
Q60. Choose the most appropriate alternative from the options given below to
complete the following sentence:
Despite several __________ the mission succeeded in its attempt to
resolve the conflict.
Answer:(B) setbacks
Q61. Wanted Temporary, Part-time persons for the post of Field Interviewer to conduct
personal interviews to collect and collate economic data. Requirements: High School-pass, must be available for Day, Evening and Saturday work.
Transportation paid, expenses reimbursed.
Which one of the following is the best inference from the above advertisement?
(A) Gender-discriminatory
(B) Xenophobic
(C) Not designed to make the post attractive
(D) Not gender-discriminatory
Answer:(C) Not designed to make the post attractive
Q62. Given the sequence of terms, AD CG FK JP, the next term is
Answer:(A) OV
Q63. Which of the following assertions are CORRECT?
P: Adding 7 to each entry in a list adds 7 to the mean of the list
Q: Adding 7 to each entry in a list adds 7 to the standard deviation of the list
R: Doubling each entry in a list doubles the mean of the list
S: Doubling each entry in a list leaves the standard deviation of the list unchanged
Answer:(C) P, R
Q64. An automobile plant contracted to buy shock absorbers from two suppliers X and Y.
X supplies 60% and Y supplies 40% of the shock absorbers. All shock absorbers are
subjected to a quality test. The ones that pass the quality test are considered reliable
Of X's shock absorbers, 96% are reliable. Of Y's shock absorbers, 72% are reliable.
The probability that a randomly chosen shock absorber, which is found to be reliable,
is made by Y is
Answer:(B) 0.334
Q65. A political party orders an arch for the entrance to the ground in which the annual
convention is being held. The profile of the arch follows the equation y = 2x - 0.1x2
where y is the height of the arch in meters. The maximum possible height of the
arch is
Answer:(B) 10 meters
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